M-MLV Reverse Transcriptase
Product Introduction
MMLV reverse transcriptase is a reverse transcriptase obtained by mutation screening of the M-MLV gene derived from Moloney murine leukemia virus and expressed in Escherichia coli. This enzyme has higher temperature tolerance, is suitable for high temperature reverse transcription, and is conducive to eliminating the adverse effects of RNA high-level structure and non-specific factors on cDNA synthesis. It has higher stability and reverse transcription synthesis ability.
Product Feature
- High temperature resistance: can perform reverse transcription at high temperatures of 50-60°C, and the reaction remains stable;
- High reverse transcription efficiency: for low-concentration RNA templates, ensure synthesis efficiency and success rate;
Product Application
- First-strand cDNA synthesis for RT-PCR and RT-qPCR
- Synthesis of cDNA for cloning and expression studies
Product Data
Comparison with SDS-PAGE electrophoresis detection of similar products
Line 1, 2, 3: CSB-DEM025 M-MLV Reverse Transcriptase
Line 4: M-MLV Reverse Transcriptase from a certain brand
Figure 1. SDS-PAGE analysis of CSB-DEM025 M-MLV Reverse Transcriptase and similar products.
Comparative testing with competitive enzymes.
Figure 2. Reverse transcription temperature test of CSB-DEM025 M-MLV Reverse Transcriptase and similar products.
Accelerated thermal stability.
Figure 3. Accelerated thermal stability of CSB-DEM025 M-MLV Reverse Transcriptase at 37°C.