One Step RT-qPCR Kit

Product Introduction

One Step RT-qPCR Kit is a universal kit for performing RT-qPCR reactions using the probe method. This kit uses RNA as a template and gene-specific primers to complete the reverse transcription and qPCR reactions in one tube, without the need for additional opening and pipetting operations, greatly increasing the detection throughput and reducing the risk of contamination. Integrating the superior performance of reverse transcriptase and hot start Taq DNA polymerase, combined with an optimized buffer system, the detection sensitivity of One Step RT-qPCR Kit can reach 0.5 pg total RNA template, and can stably and efficiently perform one-step RT-qPCR reactions.

Product Feature

- Excellent amplification efficiency and excellent detection sensitivity: compared with similar products, the Ct obtained by reverse transcription is smaller and the starting RNA amount is lower;

- Easy to use: only need to add template and primers to perform PCR amplification;

- Fast and efficient: reduce the number of sample addition steps and save time;

- Repeatable: reduce the risk of contamination and sample addition errors;

Product Application

- Rapid gene cloning

- Colony PCR

- Genotyping

- TA cloning, etc.

Product Data

Figure 1. Comparison of the sensitivity and multiple amplification tests of CSB-DKT031 One Step RT-qPCR Kit with similar products.