Taq DNA Polymerase

Product Introduction

Taq DNA polymerase is a recombinant, heat-resistant DNA polymerase. It was named Taq polymerase because it was discovered in the thermophilic aquatic bacterium Thermus aquaticus in hot springs. It has 5'-3' polymerase activity and 5'-3' exonuclease activity.

Product Feature

- High purity: purity >95%, no residual nuclease, low residual host gDNA;

- Good compatibility: compatible with multiple Buffers, active at pH 8.3-11.6;

- High sensitivity: improved template affinity through modification, increasing the detection sensitivity of low concentration templates;

- Good specificity: electrophoresis detection of target band is single, no tailing;

Product Application

- Routine PCR amplification

- RT-qPCR

- Fluorescent quantitative PCR

- Product can be directly TA cloned

Product Data

SDS-PAGE electrophoresis purity

Line 1、2、3：CSB-DEM023 Taq DNA Polymerase

Line 4：A certain brand of Taq DNA polymerase

Figure 1. SDS-PAGE electrophoresis purity detection of CSB-DEM023 Taq DNA Polymerase and similar products.

Amplification of different fragment lengths.

Line1-5：Fragment size 460/750/1500/2100/3900bp

M：Trans8K DNA Marker

Figure 2. Electrophoresis test results of CSB-DEM023 Taq DNA Polymerase.

Comparative testing with competitive enzymes.

Figure 3. qPCR was performed on plasmid templates with different copy numbers of 5000, 500, 50, and 5 copies using CSB-DEM023 Taq DNA Polymerase and similar products. It can be seen that CSB-DEM023 Taq DNA Polymerase has excellent linearity and amplification efficiency in the lower template concentration range.