HiFi DNA Polymerase

Product Introduction

HiFi DNA Polymerase is a new generation of high-fidelity DNA polymerase derived from Pfu DNA Polymerase. Compared to Pfu DNA Polymerase, the performance of HiFi DNA polymerase has been greatly improved, and it can quickly and accurately complete PCR reactions even for complex templates. Its fidelity has been significantly improved. This product comes with an optimized enzyme buffer containing PCR-enhancing components, giving the enzyme extremely high amplification efficiency and broad template adaptability, suitable for amplification of complex templates. The amplified product has blunt ends.

Product Feature

- Purity >95%, no residual nuclease, low residual host gDNA;

- Fidelity is more than 100 times higher than Taq fidelity;

- Efficient amplification of high GC content targets, poorly purified DNA and long fragment sequences;

- Pre-mixed solution with or without loading dye is available.

Product Application

- Long fragment PCR amplification

- High GC template amplification

- Cloning of DNA fragments

Product Data

Line 1-4: High GC gDNA 1K, 0.7K, 0.5K, 0.4K (GC content 60-80%).

Line 5-8: λ DNA 1K, 4K, 6K, 8K.

Figure 1. CSB-DEM042 HiFi High-Fidelity DNA Polymerase.