T4 DNA ligase

Product Introduction

T4 DNA ligase catalyzes the formation of phosphodiester bonds between adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA. The enzyme can not only catalyze the connection between blunt ends or sticky ends, but also repair single-strand nicks in double-stranded DNA, RNA, or DNA/RNA hybrid cross-chains.

Product Feature

- No residual nucleases or nickases;

- Good stability;

Product Application

- The connection of restriction endonuclease-digested fragments, Linkers or Adapters, etc., can also be used for nick repair and Ligase-mediated RNA detection.