2×Taq PCR MIX（With Dye）（A tube of）

Product Introduction

2×Taq PCR premix is optimized for conventional PCR amplification reactions. When used, just add the template and primers and dilute to 1× concentration to perform PCR reactions, greatly simplifying the operation process and reducing contamination during PCR operations. There are two forms to choose from: regular and fast-loading. Tests have shown that the addition of dye does not affect the PCR reaction, and electrophoresis can be performed directly after the PCR reaction is completed, saving time.

Product Feature

- Easy to use: Only need to add template and primers for PCR amplification.

- Repeatable: Reduces the number of sample addition steps, reducing contamination and experimental errors.

- Fast and efficient: Contains electrophoresis dye, allowing direct electrophoresis after PCR reaction, simplifying operation.

- Wide applicability: Components have been optimized for use in various conventional PCR reactions.

Product Application

- Conventional PCR amplification/identification

- Genotyping

- TA cloning

Product Data

Gel electrophoresis detection

Note: 1-3, 4-6, 7-9, 10-12, 13-15 are respectively: Primer 1, Primer 2, Primer 3, Primer 4, Primer 5. Line1, 4, 7, 10, 13: 2×Taq PCR Mix (with dye).

 Line2, 5, 8, 11, 14: 2×Taq PCR Mix (without dye, add dye after PCR).

Line3, 6, 9, 12, 15: 2×Taq PCR Mix (without dye, add regular loading buffer after PCR).

M: Trans2K DNA Marker.

Figure 1. Gel electrophoresis detection of CSB-DKT032/033 2×Taq PCR premix.

Stability test

Note: 1-4, 5-8, 9-12, 13-16, 17-20: Primer 1, Primer 2, Primer 3, Primer 4, Primer 5

Line1, 5, 9, 13, 17: Placed at 37°C for 7 days

Line2, 6, 10, 14, 18: Placed at 37°C for 14 days

Line3, 7, 11, 15, 19: Frozen at -20°C

Line4, 8, 12, 16, 20: 2×Taq PCR Mix (no dye) + dye (after PCR completion)

M: Trans2K DNA Marker

Figure 2. CSB-DKT032/033 2×Taq PCR Mix (with dye) Stability Test Comparison test with competitive kits.

Comparison test with competitor’s kit

Line1-4：CSB-DKT032       Line5-6：品牌B   M：Trans2K DNA Marker

Figure 3. CSB-DKT032 2×Taq PCR Mix (With Dye) amplifies gDNA fragments of 1-6kb,compared to brand B.