2×Taq PCR MIX（With Dye）
Product Introduction
2×Taq PCR premix is optimized for conventional PCR amplification reactions. When used, just add the template and primers and dilute to 1× concentration to perform PCR reactions, greatly simplifying the operation process and reducing contamination during PCR operations. There are two forms to choose from: regular and fast-loading. Tests have shown that the addition of dye does not affect the PCR reaction, and electrophoresis can be performed directly after the PCR reaction is completed, saving time.
Product Feature
- Easy to use: Only need to add template and primers for PCR amplification.
- Repeatable: Reduces the number of sample addition steps, reducing contamination and experimental errors.
- Fast and efficient: Contains electrophoresis dye, allowing direct electrophoresis after PCR reaction, simplifying operation.
- Wide applicability: Components have been optimized for use in various conventional PCR reactions.
Product Application
- Conventional PCR amplification/identification
- Genotyping
- TA cloning
Product Data
Gel electrophoresis detection
Note: 1-3, 4-6, 7-9, 10-12, 13-15 are respectively: Primer 1, Primer 2, Primer 3, Primer 4, Primer 5. Line1, 4, 7, 10, 13: 2×Taq PCR Mix (with dye).
Line2, 5, 8, 11, 14: 2×Taq PCR Mix (without dye, add dye after PCR).
Line3, 6, 9, 12, 15: 2×Taq PCR Mix (without dye, add regular loading buffer after PCR).
M: Trans2K DNA Marker.
Figure 1. Gel electrophoresis detection of CSB-DKT032/033 2×Taq PCR premix.
Stability test
Note: 1-4, 5-8, 9-12, 13-16, 17-20: Primer 1, Primer 2, Primer 3, Primer 4, Primer 5
Line1, 5, 9, 13, 17: Placed at 37°C for 7 days
Line2, 6, 10, 14, 18: Placed at 37°C for 14 days
Line3, 7, 11, 15, 19: Frozen at -20°C
Line4, 8, 12, 16, 20: 2×Taq PCR Mix (no dye) + dye (after PCR completion)
M: Trans2K DNA Marker
Figure 2. CSB-DKT032/033 2×Taq PCR Mix (with dye) Stability Test Comparison test with competitive kits.
Comparison test with competitor’s kit
Line1-4：CSB-DKT033 Line5-6：品牌B M：Trans2K DNA Marker
Figure 3. CSB-DKT033 2×Taq PCR Mix (With Dye) amplifies gDNA fragments of 1-6kb,compared to brand B.