Tn5 Transposase

Product Introduction

Tn5 transposase is a highly active Tn5 transposase mutant derived from E. coli that can efficiently insert Tn5 transposons randomly into target sequences, with extremely high transposition insertion efficiency for both eukaryotic and prokaryotic DNA. Tn5 transposase specifically recognizes DNA fragments with chimeric end sequences at both ends, forming a Tn5 transposon that can randomly bind to target DNA and cleave and insert its carried DNA fragment. Tn5 transposase is widely used in fields such as in vitro transgenesis and second-generation sequencing library construction.

Product Feature

- Purified multiple times by column chromatography, SDS-PAGE gel detection shows only a clear single target band;

- PCR method detects no host DNA residue;

- No contamination by endonucleases or exonucleases.

Product Application

- Transposon used in the fragmentation and adapter addition steps of constructing a second-generation sequencing library;

- In vitro or in vivo construction of a random insertion mutant library using transposons;

- Rapid sequencing of large DNA molecules (such as BAC clones);

- Introduction of resistance markers into target DNA.

Product Data

SDS-PAGE electrophoresis

Figure 1. CSB-DEM076 Tn5 Transposase SDS-PAGE Electrophoresis Image.

Product effect image

Figure 2. Effect test image of Tn5 transposon prepared by Tn5 Transposase for random DNA fragmentation.